Edvotek® Exploring Human Origin by PCR Amplification of Mitochondrial DNA
£154.50
£128.75
This kit seeks to isolate maternal DNA from cheek or hair samples. Students do the entire process, extracting and amplifying their own mitochondrial DNA with supplied primers etc.
Pack Size:
1
Estimated delivery time - 10 working days
In this experiment, students will isolate their mitochondrial DNA and use the Polymerase Chain Reaction (PCR) to amplify two separate regions of the mitochondrial genome. Results are analysed using agarose gel electrophoresis.
Group Size: For 25 students
Time Required:
Extraction - 50 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 minutes to overnight
Kit Includes: Instructions, PCR EdvoBeads™ PLUS, universal DNA buffer, TE buffer, mitochondrial LyphoPrimer™ mix, EdvoQuick™ DNA ladder, LyphoControl™, Proteinase K, UltraSpec-Agarose™, TBE electrophoresis buffer powder, SYBR® Safe stain, FlashBlue™ stain, microcentrifuge & PCR tubes, disposable plastic cups, salt packets, and a 15 mL conical tube.
All You Need:
DNA electrophoresis, thermal cycler, micropipettes: 5-50 µl, UV or blue light transilluminator, white light box (optional), (2) water baths, microcentrifuge & microwave or hot plate.
Storage: Some components require freezer storage upon receipt
Group Size: For 25 students
Time Required:
Extraction - 50 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 minutes to overnight
Kit Includes: Instructions, PCR EdvoBeads™ PLUS, universal DNA buffer, TE buffer, mitochondrial LyphoPrimer™ mix, EdvoQuick™ DNA ladder, LyphoControl™, Proteinase K, UltraSpec-Agarose™, TBE electrophoresis buffer powder, SYBR® Safe stain, FlashBlue™ stain, microcentrifuge & PCR tubes, disposable plastic cups, salt packets, and a 15 mL conical tube.
All You Need:
DNA electrophoresis, thermal cycler, micropipettes: 5-50 µl, UV or blue light transilluminator, white light box (optional), (2) water baths, microcentrifuge & microwave or hot plate.
Storage: Some components require freezer storage upon receipt
| Pack Size | 1 |
|---|---|
| Gross Weight | 0.6 |
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