Edvotek® Exploring the Genetics of Taste Kit
£195.60
£163.00
This is a great kit to use on a dedicated science activity day, exploring the science of taste and the genetics and biotechnology behind the process of identifying ‘tasters’ and ‘non-tasters’.
Pack Size:
1
In stock
Chilled
Chilled
A single nucleotide polymorphism (where only one nucleotide base has been changed) is responsible for the detection of the bitter taste on PTC (protein taste compound) paper. Here students have the opportunity of running the complete process from DNA extraction to analysing their own samples of amplified and cut DNA to detect the presence or absence of the PTC gene. This is a great kit to use on a dedicated science activity day, exploring the science of taste and the genetics and biotechnology behind the process of identifying ‘tasters’ and ‘non-tasters’.
The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.
Group Size: For 25 reactions
Time Required:
Extraction - 30 minutes
PCR Setup - 10 minutes
PCR - 2 hours
DNA Digest - 60 minutes
Electrophoresis - 30 minutes
Staining - 5 minutes
Kit Includes: Instructions, PCR EdvoBeads™, universal DNA buffer, TE buffer, LyphoPrimer™ mix, LyphoControl™ (complete PCR control), Dryzymes® restriction enzyme HaeIII, restriction enzyme dilution buffer, 100 bp ladder, Proteinase K, PTC paper, control taste paper, UltraSpec-Agarose™, TBE electrophoresis powder buffer, SYBR® safe stain, disposable plastic cups, 15 mL conical tube, snap-top microcentrifuge tubes, microcentrifuge tubes (1.5 mL screw-cap), 0.2 mL PCR tubes, and salt packets.
All You Need: DNA electrophoresis, thermal cycler, micropipettes: 5-50 µl, UV or blue light transilluminator, (2) Water Baths, microcentrifuge & microwave or hot plate.
Storage: Some components require freezer storage upon receipt
The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.
Group Size: For 25 reactions
Time Required:
Extraction - 30 minutes
PCR Setup - 10 minutes
PCR - 2 hours
DNA Digest - 60 minutes
Electrophoresis - 30 minutes
Staining - 5 minutes
Kit Includes: Instructions, PCR EdvoBeads™, universal DNA buffer, TE buffer, LyphoPrimer™ mix, LyphoControl™ (complete PCR control), Dryzymes® restriction enzyme HaeIII, restriction enzyme dilution buffer, 100 bp ladder, Proteinase K, PTC paper, control taste paper, UltraSpec-Agarose™, TBE electrophoresis powder buffer, SYBR® safe stain, disposable plastic cups, 15 mL conical tube, snap-top microcentrifuge tubes, microcentrifuge tubes (1.5 mL screw-cap), 0.2 mL PCR tubes, and salt packets.
All You Need: DNA electrophoresis, thermal cycler, micropipettes: 5-50 µl, UV or blue light transilluminator, (2) Water Baths, microcentrifuge & microwave or hot plate.
Storage: Some components require freezer storage upon receipt
| Pack Size | 1 |
|---|---|
| Gross Weight | 0.42 |
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