Edvotek® Multiplex PCR Testing of Water Contaminants Kit

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
Group Size For 25 students

Time Required:
Extraction - 60 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 mins or overnight

Kit Includes: Instructions, PCR EdvoBeads™, universal DNA buffer, LyphoPrimer™ mix, EdvoQuick™ DNA ladder, LyphoControl™ (complete PCR control), TE buffer, Proteinase K, BactoBeads™, UltraSpec-Agarose™, electrophoresis buffer (50X), SYBR® Safe stain, FlashBlue™ stain, microcentrifuge and PCR tubes.

All You Need: DNA electrophoresis, thermal cycler, micropipettes: 5-50 µl, UV or blue light transilluminator, water bath, microcentrifuge, microwave or hot plate, & isopropanol.

Storage: Some components require refrigerator or freezer storage upon receipt

BT150262 Sample Instructions