Antigen-Antibody Interaction: The Ouchterlony Procedure

Introduce your students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.
Kit includes: instructions, animal serum antigens antibodies, practice gel loading solution, agarose, powdered buffer, transfer pipettes, Petri plates, well cutters, microtest tubes.
All you need: automatic micropipets with tips, 5 or 10 ml pipettes, 55°C waterbath, measuring spatulas or toothpicks, microwave or hot plate, distilled water, incubation oven (optional).
For 10 Groups