Using CRISPR To Treat Cystic Fibrosis

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.
• Group Size: For 8 gels
• Time Required: Complete in 45 minutes
• Kit Includes: Instructions, Ready-to-load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis buffer (50X), 10X gel loading solution, FlashBlue™ DNA stain.
• All You Need: DNA Electrophoresis, micropipettes: 5-50 µl (Optional), white light box, & microwave or hotplate.
• Storage: Room temperature stable. Storage of ready-to-load QuickStrip™ samples in the refrigerator is recommended.
** A replenisher kit is available - see BT230080

BT230070 Sample Instructions