Edvotek® What Is PCR and How Does It Work? Kit

This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophoresis.

• Learn the biology behind polymerase chain reactions (PCR) and gel electrophoresis
• Explore the relationship between PCR cycle number and amplified DNA quantities
• Perform agarose gel electrophoresis to separate different sized dye molecules
• Load, run, and analyse simulated DNA samples to observe size and concentration differences
Group Size: For 10 lab groups

Time Required: Complete in 45 minutes

Kit Includes: Instructions, ready-to-load QuickStrip™ dye samples, UltraSpec-Agarose™, electrophoresis buffer (50X), practice gel loading solution, disposable pipettes.

All You Need: DNA electrophoresis, micropipettes: 5-50 µl (optional), white light box (optional), & microwave or hot plate

Storage: Room temperature stable. Storage of ready-to load QuickStrip™ samples in the refrigerator is recommended

Micro Pipetting Basics Guide Agarose Gel Electrophoresis Guide