Transformation of E. Coli with Blue & Green Fluorescent Proteins

In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.

• Explore genetic engineering in this elegant and expanded experiment
• Transform E.coli with either pFluoroGreen™ or pFluoroBlue™
• Select for transformed cells using LB-ampicillin plates and calculate transformation efficiency
• Expose transformed cells to IPTG to demonstrate differential gene expression
• Different plasmids help emphasise the concept of DNA>RNA>Protein>Trait
For 10 lab groups

Time Required: Complete in 50 minutes and grow overnight.

Kit Includes: Instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, CaCl2, growth additive, competent cell solution, ReadyPour™ agar, recovery broth, petri plates, plastic microtipped transfer pipettes, wrapped 10 mL pipette (sterile), inoculating loops (sterile), & conical tube, & microcentrifuge tubes

All You Need: Micropipettes, two water baths, floating racks or foam tube holders, thermometer, incubation oven, pipette pumps or bulbs, ice, marking pens, Bunsen burner, hot plate or microwave, hot gloves, & UV safety glasses, long wave UV light, centrifuge (optional)

Storage: Some components require refrigerator and freezer storage