Eukaryotic Cell Biology

Cell Culture is vital technology used in life science research and in biotechnology laboratories. The study of basic cell biology, diseases and cancer, the development and testing of new therapeutics, and the production of new drugs relies on using the techniques introduced in this experiment. Students will learn how to grow eukaryotic cells in culture, basic cell staining and how to count cells. The techniques used in these experiments will provide the student with skill set desired in both academic research and industry.
Kit Includes: instructions, growth media, flasks, Giemsa stain, trypan blue dye, sterile T25 flasks, sterile culture dishes, sterile large pipets, small pipets, cell counting chambers, Sf9 insect cells.
All you need: microscope and spray bottle with 70% ethanol or methanol.
For 6 Lab Groups. Basic cell culture techniques 30 min., Examination of insect cell cultures 30 min., Maintenance of insect cell cultures 1 hour, Cell Viability using trypan blue 30 min., Differential staining using giemsa stain overnight.

BT140755 Instructions